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. 2021 Nov 18;43(7):1829–1842. doi: 10.1038/s41401-021-00799-x

Fig. 8. FAK overexpression reverses the effects of ADT-OH on cell migration.

Fig. 8

a B16F10 cells were transfected with pRK5-FAK or pRK5 plasmid for 24 h and then treated with NaHS (2 mM) or ADT-OH (6.3 μM) for 24 h. The cell lysates were performed by Western blot with indicated antibodies. b The mRNA levels of FAK, E-cadherin, N-cadherin and Vimentin in B16F10 cells under different treatment conditions were examined by RT-qPCR analysis. c After transfected with pRK5-FAK or pRK5 plasmid, B16F10 cells were treated with NaHS (2 mM) or ADT-OH (6.3 μM) for 24 h. Immunofluorescence staining for FAK and F-actin was performed and representative images are shown. DAPI was used to display the nucleus of cells. d Transwell assay. B16F10 cells were transfected with pRK5-FAK or pRK5 plasmid and then exposed to NaHS (2 mM) or ADT-OH (6.3 μM). After 24 h, the cells that migrated to the inferior membrane were stained and counted in five fields at a magnification of ×100. n = 5, bar = 100 μm. The experiments were carried out in triplicate and representative data are shown. *P < 0.05, **P < 0.01, ***P < 0.005