FIGURE 5.

SHY-01 administration displays neuronal protection post-ischemia. SHY-01 (SHY, 50 mg/kg) or metformin hydrochloride (Met, 50 mg/kg) was administrated (i.p.) every 24 h post-reperfusion, saline was used as vehicle control, rats were sacrificed at 72 h after tMCAO, and brains were assessed for immunofluorescence analysis with frozen sections. (A) Apoptotic cells in the ischemic striatum were stained with TUNEL (red) as detailed in the Methods, nuclei were stained with Hoechst (blue), scale bar 50 μm. (B) Percentage of TUNEL-positive apoptotic cells among total cells were determined with ImageJ software and statistical analysis were presented. Data are expressed as mean ± SD, n = 5, statistical analysis was performed with one-way ANOVA followed by a Dunnett’s post hoc tests. **p < 0.01 versus the tMCAO with vehicle treatment group, ^## p < 0.01 tMCAO treated with Met versus SHY-01-treated group.