FIGURE 6.

SHY-01 administration suppresses microglia activation in the ischemic striatum. SHY-01 (SHY, 50 mg/kg) or metformin hydrochloride (Met, 50 mg/kg) was administrated (i.p.) daily post reperfusion, saline was used as vehicle control, rats were sacrificed at 72 h after tMCAO. (A) Brains were assessed for immunofluorescence analysis with frozen sections. Microglia were marked with Iba-1 (green) and nuclei were stained with Hoechst (blue). Representative images detected with confocal microscopy show cellular morphology in the ischemic striatum, n = 4 rats per group, scale bar: 20 μm. Brains were lysed with Trizol to extract total message RNA, then were reverse transcribed to determine mRNA level of (B) TNF-α, (C) IL-1β, and (D) iNOS. GAPDH was used as interior reference. Data were expressed as mean ± SD, n = 5 per group, statistical analysis using one-way ANOVA followed by Dunnett’s post hoc tests. ^∗∗ p < 0.01 versus the tMCAO with vehicle treatment group, ^## p < 0.01 versus the tMCAO with SHY treatment group.