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. 2022 Jun 21;13:890073. doi: 10.3389/fimmu.2022.890073

Figure 4.

Figure 4

Reduced CSR but relatively normal plasma cell differentiation in patients’ B cells. Purified naïve B cells were cultured for 6 days in medium alone or in the presence of the indicated stimuli and analyzed for B cell survival, Ig gene CSR, plasma cell differentiation and antibody secretion. (A) B cell viability. (B) Live B cell numbers. (C, D) The proportions of IgG+ B cells in freshly isolated naïve B (day 0) and naïve B cells cultured for 6 days with CD40L + IL-4. Representative FACS profiles (C) and results of 3 HC and 4 patients (D) are shown. (E–G) In vitro PB differentiation. (E) Representative FACS profiles of CD19+ cells in PBMC (day 0) and in naïve B cells cultured for 6 days with TI or TD stimuli. (F) Proportion and (G) number of plasmablasts in HC and the patients. (H and I) Naïve B cells were cultured under the indicated conditions for 6 days and the levels of IgG (H) and IgM (I) in the culture supernatants were measured by ELISA. (J, K) IgG (J) and IgM (K) secretion on a per PB basis. The amount of IgG (H) or IgM (I) in the culture supernatants of TI stimuli was divided by the total number of PB shown in (G). HC, n=2-3; Patient, n=2-4. *p < 0.05; **p < 0.01 (unpaired t-test).