Figure 3.

mRNA expression analysis of (A) vitellogenin receptor (HlVgR) and (B) autophagy-related gene 6 (HlATG6) in the ovary after semiartificial mouse skin membrane feeding with bovine red blood cells (RBC group) or with Babesia-infected bovine red blood cells (Babesia-iRBC group). Relative expression levels from extracted total RNA were determined by real-time PCR as shown in Figure 2 . Data represent the mean ± SD of triplicated samples. Asterisks indicate the significant differences between the RBC and Babesia-iRBC groups (*P < 0.05, **P < 0.01 using the Tukey–Kramer method).