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. 2022 Jul 4;13:3842. doi: 10.1038/s41467-022-30652-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Slice from a tomographic volume acquired in a frozen-hydrated human macrophage revealing the podosome environment. Colored arrows point to ribosomes (gray), glycogen granules (white), a microtubule (black), and intermediate filaments (brown). See also Supplementary Movie 3. The original tomogram has been deposited in the EMDB under accession code EMD-13671. Nine other tomograms showing similar organizations were obtained. b Orthographic view of the corresponding 3D segmentation of the actin filaments showing their relative orientation with respect to the basal membrane, intermediate filaments (brown), and organelle membranes (gray). c, d Perspective views of the actin filaments in the volume indicated by a dotted rectangle in b shown from the left (c) and right (d) sides. e–g Average filament density (e), orientation (f), and length (g) as a function of the radial distance from the core center for ten tomograms. Inset in f: Corresponding values estimated for the core radius (Methods). The podosome shown in a–d corresponds to #9. Source data are provided as Source Data files.