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. 2022 Jul 4;7:75. doi: 10.1038/s41541-022-00503-y

Fig. 4. A schematic overview of the lentiviral vector system.

Fig. 4

The HIV-1 virion and genome are included in the top panel for reference. a Transfer Vector Plasmid: this plasmid combines the 5’ and 3’ long terminal repeats (LTRs), and psi component of the HIV-1 genome, along with a promoter, transgene, and the woodchuck hepatitis virus regulatory element (Wpre). The full deletion of the U3 (unique 3’ end) region in the 5’ LTR and partial deletion of the U3 region in the 3’ LTR renders the vector self-inactivating (SIN). b Envelope plasmid: this plasmid contains a promoter to drive the expression of the VSV-G envelope protein (env) used to pseudotype lentiviral vector particles. c Packaging plasmid: This plasmid contains a promoter to drive expression of the group specific antigen (gag), DNA polymerase (pol), rev, and trans-activator of transcription (tat) elements of the HIV-1/SIV genome.