Skip to main content
. 2022 Jun 21;10:925971. doi: 10.3389/fcell.2022.925971

FIGURE 2.

FIGURE 2

Macrophages co-cultured with dying adipocytes (dAC) upregulate Cx43 expression (A–B) Immunoblot analysis of Cx43 protein (A) and qPCR analysis of Gja1 transcript levels (B) in F4/80+ macrophages isolated from gWAT co-cultured with dying adipocytes (dAC) for 24 h (A: n = 6, B: n = 3) (C) Immunofluorescence images of Cx43 staining in F4/80+ macrophages from gWAT co-cultured with or without dying adipocytes (dAC) for 24 h (n = 3). Unpaired, two-tailed t-tests (*p < 0.05, ***p < 0.001), each point represents biological replicate. Data are presented as mean ± S.E.M.