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. 2022 Jun 21;10:925971. doi: 10.3389/fcell.2022.925971

FIGURE 5.

FIGURE 5

Macrophage-specific Cx43 KO protected mice from HFD-induced inflammation in gonadal white adipose tissue (A) qPCR analysis of gWAT of WT and Cx43 MKO mice fed with either normal chow diet (NCD) or high-fat diet (HFD) for 8weeks (n = 6). Significant effects of genotype (Gja1: p = 0.001, P2rx4: p < 0.0001, P2rx7: p < 0.0001, Ccl2: p < 0.0001, Il-1b: p < 0.0001, Il-6: p < 0.0001, Il-10: p < 0.0001) and significant effects of diet (Gja1: p < 0.0001, P2rx4: p < 0.0001, P2rx7: p < 0.0001, Ccl2: p < 0.0001, Il-1b: p = 0.0039, Il-6: p < 0.0001, Il-10: p = 0.0391) were observed. Significant differences between WT HFD and KO HFD groups were determined by Bonferroni post hoc tests (B) Representative images of H/E and F4/80 stained paraffin sections, and quantification of F4/80 fluorescence intensity in gWAT of WT and Cx43 MKO mice fed NCD or HFD for 8 weeks (n = 4). Significant effects of genotype (p < 0.0001) and diet (p < 0.0001) were observed. Significant differences between WT HFD and KO HFD groups were determined by Bonferroni post hoc tests (C) Immunoblot analysis of gWAT of WT and Cx43 MKO mice fed with either NCD or HFD for 8 weeks (n = 6). Significant effects of genotype (Cx43: p < 0.0001, F4/80: p < 0.0001, p-RIP3: p < 0.0001) and significant effects of diet (Cx43: p < 0.0037, F4/80: p = 0.003, p-RIP3: p < 0.0001) were observed. Significant differences between WT HFD and KO HFD groups were determined by Bonferroni post hoc tests. Two-way ANOVA with Bonferroni post hoc tests was used (***p < 0.001). Each point represents biological replicate. Data are presented as mean ± S.E.M.