REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal to VCAM-1	ABCAM	Cat. #Ab134047; RRID: AB_2721053
Mouse monoclonal to E-selectin	Thermo Fisher Scientific	Cat. #MA1-34486; RRID: AB_1955403
Goat anti-mouse IgG secondary Ab, Alexa Fluor 568	Thermo Fisher Scientific	Cat. #A-11031; RRID: AB_144696
Goat anti-rabbit IgG secondary Ab, Alexa Fluor 488	Thermo Fisher Scientific	Cat. #A-11034; RRID: AB_2576217
Biological samples
Healthy adult whole blood	Adult Volunteers	No stored samples or data
Iron deficiency anemia patient whole blood	Emory University School of Medicine Department of Hematopathology	De-identified patient samples in Redcap
Chemicals, peptides, and recombinant proteins
Cell-Tak Cell and Tissue Adhesive	Corning	Cat. # 354240
Sylgard 184 (PDMS)	Ellsworth Adhesives	Cat. # 4019862
Paraformaldehyde 16%	Thermo Fisher Scientific	Cat. # 50-980-487
EGM-2 growth media	Lonza	Cat. #CC-3162
Bovine Serum Albumin	Sigma-Aldrich	Cat. # A7906-50G
Deposited data
Raw and analyzed data	This paper; Mendeley data	[Mendeley]: [https://doi.org/10.17632/gtv74r5zt5.1].
Experimental models: Cell lines
Human umbilical vein endothelial cells (HUVECs)	Lonza	Cat. #CC-2519
Software and algorithms
ImageJ version 1.53a	National Institute of Health	http://imagej.nih.gov/ij
GraphPad Prism version 9.2.0	GraphPad Software	www.graphpad.com
Custom Hematology Analysis Software	The Lam Lab; This paper	www.github.com/iCLOTS
AFM Hertzian contact model	PYTHON	www.github.com/nstone8/pyrtz
Other
EDTA blood collection tubes	Beckton Dickinson	Cat. #367856
Atomic Force Microscopy	Asylum Research	Model: MFP-3D Bio-AFM
AFM Fluorodish	World Precision Instruments	Cat. #FD35-100
Syringes	Beckton Dickinson	Cat. #309628
Syringe Pump	Harvard Apparatus	Cat. #70-3007
Inverted Optical Microscope	Nikon	Model: Eclipse TE2000-U