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. 2022 Jun 21;10:890882. doi: 10.3389/fbioe.2022.890882

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Copyright © 2022 Miao, Niibe, Fu, Zhang, Nattasit, Ohori-Morita, Nakamura, Jiang and Egusa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Establishment of mouse Epfn-iPSCs. (A) Generation of doxycycline-inducible Epfn-expressing piggyBac vector (PB-Epfn). (B–D) Inducible Epfn expression in Epfn-iPSCs after 24 h of culture with different doses of Dox (0–2 μg/ml) was examined by real-time PCR (B) and Western blot (C) along with mCherry expression (D). (E,F) Epfn-iPSCs (E-iPSCs) showed comparable pluripotency to naive iPSCs according to the expression of pluripotent marker genes (E), ALP staining, and immunofluorescence results for SSEA-1 and Nanog (F). Scale bar: 100 μm.