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A
CBX4‐SUMOylated candidates formed a large STRING network, which were further clustered into 12 sub‐clusters utilizing k‐means clustering. These 12 interconnected sub‐clusters are shown in different colors.
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B
SUMOylated proteins were proceeded to Gene Ontology (GO) analysis utilizing PANTHER classification system, which were classified into 21 functional classes.
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C–F
Four highly interconnected STRING networks including mRNA splicing complex, PcG protein complex, NuRD complex and activation of SUMOylation.
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G
HA‐tagged EZH2 was co‐overexpressed with Flag‐tagged SUMO1, Flag‐tagged UBC9 or Flag‐tagged CBX4, and siNC. In the last group, HA‐tagged EZH2 was co‐overexpressed with Flag‐tagged SUMO1, Flag‐tagged UBC9 and siCBX4. EZH2 was IP with anti‐HA beads. Both total and IP samples were IB with antibodies against HA, Flag and GAPDH. The expression ratios of SUMO1‐EZH2 within each group are shown below the figure.
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H
HA‐tagged EZH2 was co‐overexpressed with Flag‐tagged SUMO4, Flag‐tagged UBC9 or Flag‐tagged CBX4, and siNC. In the last group, HA‐tagged EZH2 was co‐overexpressed with Flag‐tagged SUMO4, Flag‐tagged UBC9 and siCBX4. EZH2 was IP with anti‐HA beads. Both total and IP samples were IB with antibodies against HA, Flag and GAPDH. The expression ratios of SUMO4‐EZH2 within each group are shown below the figure.
