FIGURE 3.

SUMO1‐mediated SUMOylation of Kir7.1 was upregulated in the spinal cord after SNI. (A) Upreguation in SUMO1 was immunoprecipitated with Kir7.1 antibody on Day 10 after SNI (n = 6 rats per group. IgG, immunoglobulin G; IB, immunoblot). (B) Co‐immunoprecipitation (IP) of SUMO2/3 with antibody to Kir7.1 in lysates from spinal cord tissues isolated from rats at 10 days after SNI or the sham group (n = 6 rats per group). (C) Immunohistochemistry assessing the colocalization of Kir7.1 with SUMO1 in spinal cord tissues in normal rats (n = 3 rats, Scale bar, 100 μm). (D) Details of the SUMO‐conjugation motifs and summary of prediction software scores. (E) Alignment of Kir7.1 sequences from different species as indicated at the consensus SUMOylation site