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. 2022 May 19;23(7):e54992. doi: 10.15252/embr.202254992

Figure 4. Behavior of meiosis‐regulating proteins in spermatocyte‐injected oocytes.

Figure 4

  1. SGO2 localizes at the centromeres of spermatocyte‐derived and maternal chromosomes. Oocytes were fixed at metaphase I and stained for SGO2 (green), ACA (centromeres, magenta), and Hoechst33342 (DNA, blue). Images were reconstructed in 3D and viewed from the top and side of the metaphase plate. Note that signals are interpolated in z.
  2. PLK1 localizes at the kinetochores of spermatocyte‐derived and maternal chromosomes. PLK1 (green) localization was investigated as in (A).
  3. MAD2 kinetochore localization is defective on spermatocyte‐derived chromosomes. The localization of the closed (active) form of MAD2 (C‐MAD2, green) was investigated as in (A). Note that the kinetochore enrichment of MAD2 was less on the chromosomes in half of the metaphase plate, which likely corresponded to spermatocyte‐derived chromosomes, in control and halved oocytes.
  4. Effect of forced SAC activation. Oocytes expressing MAD1‐mNeonGreen‐CENP‐C (green), together with major satellite–mClover (green) and H2B–mCherry (red), were imaged. Time after the start of imaging (h:mm) is shown. Oocytes were categorized based on anaphase figures (n = 24, 23 oocytes).