FIG. 5.

Effect of DmpR mutations on growth rates with 4-methylphenol. The rates of CO₂ roduction by the indicated strains were measured upon growth on minimal medium supplemented with 2.5 mM 4-methylphenol. The rate achieved for each original mutant isolate of CF600.1::Km-gfp is compared with that of a regenerated CF600 derivative harboring the detected DmpR mutation. The profiles for CF375.3::Km-gfp (closed squares) and CF600.1::Km-gfp (open squares) are also shown to aid comparison. Values are the averages from duplicate experiments. Results similar to those shown in panel A were obtained with strains harboring DmpR-R109C,-L113V, -D116N, -F122L, and -179(CG) 180 (data not shown).