TABLE 2.
Effector response profiles of DmpR derivatives in KT2440::Po-luxAB
| Derivative | Luciferase activity witha:
|
|||||
|---|---|---|---|---|---|---|
| 4-MPb | 3, 4-DMPb | 2, 4-DMP | 4-EP | 4-NP | 2, 4-DCP | |
| Wild type | ++ | ++ | − | − | − | − |
| F42Y | +++ | +++ | ++ | − | − | +++ |
| R109C | +++ | ++ | ++ | + | ++ | +++ |
| L113V | +++ | +++ | + | − | − | +++ |
| D116N | +++ | +++ | ++ | + | ++ | +++ |
| F122L | ++ | ++ | ++ | ++ | − | +++ |
| E135K | +++ | +++ | ++ | ++ | +++ | +++ |
| C137Y | ++ | ++ | − | − | − | − |
| 179(CG)180 | ++ | ++ | − | − | − | − |
a
All compounds were present at a final concentration of 0.5 mM. Abbreviations: MP, methylphenol; DMP, dimethylphenol; EP, ethylphenol; NP, nitrophenol; DCP, dichlorophenol. Plus symbols represent the intensity of the luciferase activity as observed on film, a minus symbol indicates no activity above background in the absence of any aromatic effector.
b
Compounds can serve as a growth substrate.