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. 2022 Jan 1;40(3):380–398. doi: 10.5534/wjmh.210164

Table 5. Summary of response from 28 experts recommending ASA testing in male infertility patients.

Variable Value
Indications for ASA testinga
Sperm agglutination 24 (85.7)
Asthenozoospermia 15 (53.6)
Failed IUI 13 (46.4)
Failed IVF 13 (46.4)
Other 4 (14.3)
Antisperm antibody tested
IgA 3 (10.7)
IgG 4 (14.3)
Both 21 (75.0)
Technique for testing
MAR test (indirect) 18 (64.3)
Immunobead test (direct) 8 (28.6)
Other 2 (7.1)
Relevance of ASA testing
Effective 20 (71.4)
Neutral 7 (25.0)
Very effective 1 (3.6)
Biological fluid tested for ASAa
Semen 22 (78.6)
Seminal plasma 8 (28.6)
Serum 4 (14.3)
Cut-off value for abnormal ASA testing (%)
>20 10 (35.7)
>40 10 (35.7)
>50 8 (28.6)
Frequency of the ASA test ordered
Monthly 12 (42.9)
Yearly 9 (32.1)
Weekly 7 (25.0)
Number of ASA testing ordered monthly
1–5 24 (85.7)
6–9 0 (0.0)
10–15 2 (7.1)
>15 2 (7.1)
Initial recommendation in case of positive ASA test
Steroids 14 (50.0)
ART 8 (28.6)
Sperm washing for IUI 2 (7.1)
Other 4 (14.3)

Values are presented as number (%).

ASA: antisperm antibodies, IUI: intrauterine insemination, IVF: in vitro fertilization, IgA: immunoglobulin A, IgG: immunoglobulin G, MAR: mixed antiglobulin reaction, ART: assisted reproductive technology.

aMultiple options can be selected.