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. Author manuscript; available in PMC: 2022 Jul 5.
Published in final edited form as: Clin Sci (Lond). 2022 Jun 17;136(11):879–894. doi: 10.1042/CS20220117

Figure 2. SSHTN increased lymphatic vessel density in the gonads.

Figure 2.

Lymphatic vessel density in (A) testes and (B) ovaries from control and SSHTN mice as determined by LYVE-1+ pixels relative to DAPI per field. Representative images of LYVE-1 immunofluorescence in testis (scale bar = 100 μm) and ovary (scale bar = 200 μm) sections (n=6 in both males and females). Green: LYVE-1; Red: CD31; Blue: DAPI. Gene expression of lymphatic vessel markers in (C) testes and (D) ovaries from the control and SSHTN mice (n=6 in both males and females). Results are expressed as mean ± SEM, and statistical analyses consisted of Student’s t test. *P<0.05 vs control. Representative 3-D model of confocal images of clear, unobstructed brain/body imaging cocktails and computational analysis (CUBIC) cleared testis immunostained with LYVE-1 from (E) control and (F) SSHTN mice, n=3. Representative 3-D model of confocal images of clear, unobstructed brain/body imaging cocktails and computational analysis (CUBIC) cleared ovary immunostained with LYVE-1 from (G) the control and (H) SSHTN mice, n=3. Scale bars = 1000 μm (testis) and 500 μm (ovary).