FIG. 2.
Efficiency of VNA recovery from environmental samples by the GuSCN-silica bead extraction method. Concentrated samples from either San Diego River (A) or Moonlight Creek (B) were seeded with adenoviruses to a final concentration of 105/μl. Lane 1, negative control; lanes 2 to 7, seeded samples were diluted from 100 to 10−5 in seawater concentrates and detected by nested PCR; lanes 8 to 13, seeded environmental samples were extracted by the GuSCN-silica bead method and diluted with sterilized DI water from 100 to 10−5, respectively; lane M, 25-bp molecular weight ladder.