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. 2022 Jun 24;16:100334. doi: 10.1016/j.mtbio.2022.100334

Fig. 8.

Fig. 8

The effect of 3D bioprinted double-layered skin substitutes on HUVECs and macrophages (A) ELISA results of PDGF-BB release. (B) The laser confocal micrograph of an in vitro 3D bioprinted double-layered substitute. Scale bar: 200 ​μm. (C) Transwell migration assay of HUVECs co-cultured with 3D bioprinted substitutes. Scale bar: 100 ​μm. (D)Tube formation assay of HUVECs co-cultured with 3D bioprinted substitutes. Scale bar: 200 ​μm. (E) Immunofluorescent staining of Raw 264.7 macrophages co-cultured with 3D bioprinted substitutes. Scale bar: 200 ​μm. Data are expressed as the mean ​± ​SEM; n ​= ​3; ∗∗P ​< ​0.01; ∗∗∗∗P ​< ​0.0001.