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. 2022 Jun 24;63:101531. doi: 10.1016/j.molmet.2022.101531

Figure 1.

Figure 1

Identification of CLSTN3 expression in human adipose tissue. (A and B) Distribution of RNA sequencing reads in the Clstn3 locus from inguinal white adipose tissue (iWAT) of mice (A), and in the CLSTN3 locus from subcutaneous white adipose tissue (SAT) of humans (B). (C) Relative mRNA expression of adipocyte marker gene PPARG and LEP assessed by quantitative PCR in stromal vascular fraction (SVF) and mature adipocyte fraction isolated from human SAT biopsies (n = 5). (D) CLSTN3 mRNA expression in SVF and adipocytes isolated from human SAT biopsies (n = 5). (E) Immunoblot analysis of CLSTN3 protein expression in membrane fraction and cytosolic fraction isolated from human adipocytes; CAV1, membrane protein marker; Actin, cytosolic protein marker. Data are presented as mean ± standard error of the mean (SEM), and ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.