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. 2001 Nov 15;29(22):4570–4580. doi: 10.1093/nar/29.22.4570

Figure 1.

Figure 1

Recruitment of the RNA pol II holoenzyme component Gal11P to DNA stimulates replication from a chromosomal ARS1 site. (A) Two-dimensional gel analysis of chromosomal ARS1 replication intermediates generated in the RL1 yeast strain expressing Gal4(1–100) together with either Gal11 or Gal11P. The schematic structure of the modified ARS1 locus located in the 4.7 kb genomic NcoI restriction fragment is shown at the top. The indicated bubble arc is formed by replication intermediates that initiated from ARS1, while the Y arc is mostly generated by a replication fork that initiated at neighboring origins (see text for more details). (B) Two-dimensional gel analysis of chromosomal ARS1 replication intermediates generated in the RL1 yeast strain expressing Gal4(1–94) or Gal4–CTF (the Gal4 DNA-binding domain fused to the activation domain of CTF). The position of the bubble arc is indicated by an arrow.