Figure 3.

Vascularisation is improved and distal fibrosis is reduced in EV-Treated animals. (A) Representative immunohistofluorescence images of IsolectinB4 (IsoB4; green), elastin (red), cardiac troponin I (cTnI; white) and DAPI (blue) in the infarct core at 30 days post-MI. (B) Percentage of vessel area within the infarct core (left) and scaffold (right). (C) Representative microphotographs of Picrosirius Red staining distinguishing the collagen (red) and cardiac muscle (yellow) under bright field (upper panels), and polarized light (bottom) exhibiting collagen I (red/yellow) and collagen III (green) fibrils in the same sections of distal myocardium of each experimental group. (D) Percentage of collagen I, III and col I/III ratio in distal (upper panel) and infarct (bottom) areas of the 3 groups of the study. Tukey boxplots from n = 4;7;8 animals in Untreated, Control and EV-