MiR-155 deletion ameliorates cisplatin-induced AKI in vivo. (A) Real-time PCR analysis of miR-155 levels in wild type and mice kidneys 72 h after cisplatin administration. wild type (n = 3); WT+ Cis (n = 3). (B and C) Representative immunofluorescence double staining of AQP1 (green, proximal tubules) and miR-155 (red) in kidneys of mice. Scale bar: 50 µm (D) Body weight (0 vs. 72 h), (E) Survival after cisplatin injection was monitored until day 3 (n =7). (F) Serum creatinine levels and BUN (G) over time, 72 h after saline or cisplatin administration (n = 7). (H and I) Representative kidney histology as shown by PAS staining 72 h after saline or cisplatin injection. Scale bar: 200 µm. The dot plot showed the corresponding quantification of tubular injury score (n = 7). Data are presented as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.