MiR-155 inhibition reduces cisplatin-induced DNA damages both in vivo and in vitro. (A) Representative immunofluorescence double staining of LTL (green, proximal tubules) and γH2AX staining (red) in kidney tissues. Scale bars: 50 µm. (B) The quantification of γH2AX + TECs (n = 7). (C and D) Representative western blot gel documents and summarized data showing the protein levels of γH2AX in the kidneys of mice with cisplatin-induced AKI. (E and F) Representative images of γH2AX staining in HK-2 cells and quantification of the γH2AX positive cells per field (n = 10). Scale bars, 20 µm. (G and H) Representative western blot gel documents and summarized data showing the protein levels of γH2AX in HK-2 cells treated with cisplatin (n = 3). Data are presented as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.