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. 2022 Jun 6;12(10):4753–4766. doi: 10.7150/thno.72456

Figure 5.

Figure 5

MiR-155 deletion ameliorates cisplatin-induced telomeric DNA damage by enhancing TRF1. (A) The schematic diagram depicted the predicted binding site of has-miR-155 targeting the 3'-UTR of TRF1. (B) Luciferase reporter assay determined TRF1 as a bona fide target of miR-155 (n = 3). (C-E) Western blotting of γH2AX and TRF1 in HK-2 cells (n = 3). HK-2 cells were treated with cisplatin 0, 5, 10, 20 µM for 24 h. (F-H) Western blotting of γH2AX and TRF1 in HK-2 cells (n = 3). HK-2 cells were treated with cisplatin 20 µM for 0, 12, 24, 36 h. (I and J) Representative images of TRF1-stained kidney sections from wild type and miR-155-/- mice 72 h after saline or cisplatin injection(n = 7). Scale bars: 50 µm. (K and L) Western blotting of TRF1 in kidney tissues from wild type and miR-155-/- mice 72 h after saline or cisplatin injection (n = 3). (M and N) Western blotting of TRF1 in HK-2 cells (n =3). (O and P) Representative images of TRF1-stained in HK-2 cells treated with cisplatin or saline for 24 h (n = 10). Scale bars: 20 µm. (Q) Representative images of γH2AX-and TRF1-stained sections of HK-2 cells. Scale bars: 2 µm. (R-T) Western blotting of γH2AX and TRF1 in HK-2 cells (n = 3). Data are presented as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.