Figure 2.

Reprogramming astrocytes into neurons in response to Notch1 inhibition in vitro. (A and B) After Notch1 was inhibited by shRNA (A) or DAPT (B), the expression of representative genes for neurogenic factors in astrocytes was determined by qRT-PCR analysis at the indicated time. The expression of these neurogenic factors in neurons was served as a positive control. (C) Experimental procedures. (D and E) Notch1 knockdown by shRNA induced astroyctes reprogramming into neurons. (F and G) Pharmacological inhibition of Notch1 by DAPT induced astroyctes reprogramming into neurons. **p < 0.01, ***p < 0.001 by one-way ANOVA with Tukey's post hoc test (n = 3 for each group). The scale bars represent 50 µm.