Figure 2.

L. Mix treatment increased total body BMD and lean mass in male mice. Ten-week-old male mice were treated with either vehicle (veh) or a mixture of three lactobacilli (L. Mix) for 6 weeks, starting 2 weeks before orchiectomy (orx) or sham surgery. At the end of the experiment, mice were analyzed with whole-body DXA to measure (A) percentage of fat, (B) lean mass and (C) areal bone mineral density (BMD) of whole body. Symbols in the scatter plots represent individual mice and the bars indicate mean ± s.e.m. (n = 13–15). The overall effects of treatment (Veh/L. Mix), surgical procedure (sham/orx) and their interaction were calculated using two-way ANOVA, NS, not significant. The effect of L. Mix compared to veh treatment in sham and orx mice, respectively, was calculated by Sidak’s post hoc test to correct for multiple comparisons, *P ≤ 0.05.