Table 2.

Types of diagnostic tests done in confirmed cases of Cushing’s syndrome.

Diagnosis	Diagnostic investigations	Confirmatory test
Cushing’s disease (first presentation)
1	LNSC, OST, LDDST	Positive histology, Post op AI
2	LNSC, LNS cortisone, ODST, UFC	Positive histology
3	LNSC, LNS cortisone, LDDST, ODST	Positive histology
4	LNSC, LNS cortisone, LDDST, ODST, UFC	Positive histology
5	LNSC, LNS cortisone, LDDST, UFC,	Positive histology, Post op AI
6	LNSC, LNS cortisone, LDDST, ACTH	Positive histology, Post op AI
7	LNSC, LNS cortisone, LDDST, ODST, UFC	Positive histology, Post op AI
8	LNSC, LNS cortisone, LDDST, ODST	Positive histology, Post op AI
9	LNSC, LNS cortisone, LDDST, ODST, UFC	Positive histology, Post op AI
10	LNSC, LNS cortisone, LDDST, ODST, UFC	Positive histology, Post op AI
Recurrent Cushing’s disease
11	LNSC, LNS cortisone, LDDST	Positive histology
12	LNSC, LNS cortisone, ODST	Positive histology
13	LNSC, ODST	Positive histology
14	LNSC, LNS cortisone, ODST	Positive histology
15	LNSC, LNS cortisone, ODST	Positive histology
16	LNSC, LNS cortisone, LDDST, ODST	Positive histology
Arenal Cushing’s syndrome
17	LNSC, LNS cortisone, ODST	Adrenocortical cancer
18	LNSC, LNS cortisone, LDDST, UFC	Adrenocortical cancer
19	LNSC, LNS cortisone, LDDST, ODST	Cortical adenoma
20	LNSC, LNS cortisone, LDDST	Cortical adenoma
Ectopic ACTH-secreting Cushing’s syndrome
21	LNSC, LNS cortisone, LDDST, ODST	DIPNECH with ectopic ACTH

LNS cortisone, late-night salivary cortisone; LNSC, late-night salivary cortisol; LDDST, 48-h low-dose dexamethasone suppression test; ODST, overnight dexamethasone suppression test; UFC; 24-h urinary free cortisol.