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. 2022 Jul 1;82:104125. doi: 10.1016/j.ebiom.2022.104125

Figure 6.

Figure 6

MHC binding affinity of initiating-cell specific neoantigen peptides of AHNAK2, PTPRF and NPIPA5 gene and their corresponding wild peptides. Control 1 is the beads captured QuickSwitchTM Tetramer-PE for adjusting compensation. Control 2 is the beads that have not captured any tetramer-PE and no Exiting Peptide-FITC. The low MFI (mean fluorescence intensity) FITC15.4 corresponds to 100% peptide exchange. Control 3 is the beads that have captured Tetramer-PE and Exiting Peptide-FITC. MFI FITC483 corresponds to 0% peptide exchange. The reference peptide with MFI FITC 38.7 serves as a positive control for peptide exchange of the tetramer. The higher MFI FITC in neoantigens vs. that in corresponding wild type peptides meaning the higher frequency of peptide exchange and higher MHC binding affinity of neoantigens. The sequence of each peptide and the percentage of peptide exchange was shown in Table 1.