Figure 4.

IL-6-induced Glut5 expression promotes tumorigenesis. (A) HSC-3 cells were stably expressed with distinct Glut5 shRNAs. Immunoblots were performed with indicated antibodies. (B) 50 HSC-3 cells stably expressed with control shRNA or Glut5 shRNA were seeded, and continuously cultured for 14 days in presence or absence of 50 ng/mL IL-6. The clone numbers were counted. **P < 0.01. (C) HSC-3 cells with stable expression of IL-6 or Glut5 shRNA were subcutaneously injected in mice. The images of two representative xenografts from each group were shown (left panel). The volume of mice tumor xenografts (n = 7) was measured at indicated time points after injection (right panel). Data represent the mean and SD. ***P < 0.001. (D) Immunohistochemical staining of mouse xenografts samples was performed with indicated antibodies. Scale bar, 50 µm.