NET-N lysis buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris-HCl pH 8.0 | 10 mM | 302.9 mg |
| NaCl | 100 mM | 1.5 g |
| Glycerin | 10% | 25 mL |
| EDTA | 1 mM | 93.1 mg |
| IGEPAL® CA-630 | 0.5% | 1.25 mL |
| Add ddH2O to | n/a | 250 mL |
Store at 4°C for 6 months.
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