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6× sample buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris-HCl pH 6.8 | 375 mM | 1.4 g |
| SDS | 12% | 3.6 mL |
| Glycerin | 30% | 9 mL |
| DTT | 500 mM | 2.3 g |
| Bromophenol blue | 0.01% | small spatula tip (solution becomes midnight blue) |
| Add ddH2O to | n/a | 30 mL |
Aliquot 1 mL buffer in 1.5 mL centrifugation tubes and can be stored at −20°C for up to 1 year.
