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. 2022 Jun 15;13:874778. doi: 10.3389/fimmu.2022.874778

Figure 2.

Figure 2

IL-2c effectively expands Tregs in the skin. (A, B) Six- to 12-week-old female C57BL/6 mice were intraperitoneally injected with IL-2 (1 μg per 18 g), IL-2 Fc (2.67 μg per 18 g), IL-2c (1 μg of IL-2 and 5 μg of 5344.111 per 18 g) on days 0, 1, and 2, and analyzed on day 4. Representative FACS plots show Tregs in the skin (A), and graphs show quantification of Tregs in indicated organs (B). (C, D) Six- to twelve-week-old female C57BL/6 mice were IL-2c injected on days 0, 1, and 2 and analyzed on day 4. Experimental schemes are shown (C, top), and representative dot plots show skin Tregs in PBS- or IL-2c-injected mice (C, bottom). Graphs show quantification of Tregs in the skin and CD8 T cells in PBS- or IL-2c-injected mice (n = 3–6) (D). (E) Representative images of skin Tregs of PBS- or IL-2c-injected mice harvested on day 4. Arrows depict Tregs. (F, G) Graphs show Treg ratios between IL-2c-injected and non-injected mice in indicated organs (F) and kinetics of skin Tregs of PBS- or IL-2c-injected mice (G). Results are from two independent experiments. Horizontal bars indicate mean values, error bars indicate SD, and each dot represents an individual mouse (B, D). An unpaired two-tailed t-test was used. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, non-significance (p > 0.05).