Figure 3.

Intradermal injection of IL-2c efficiently expands Tregs in C3H/HeJ mice. Female C3H/HeJ mice were intraperitoneally or intradermally injected with IL-2c on days 0, 1, and 2 and analyzed on day 4. (A) Representative dot plots show skin Tregs among total CD4 T cells pre-gated on live CD45.2⁺CD11b¯B220¯TCRβ⁺CD8¯ cells. (B) Graphs show the frequencies and absolute numbers of Tregs expressing indicated markers in each group (n = 3). Horizontal bars indicate mean values, error bars indicate SD, and each dot represents an individual mouse. An unpaired two-tailed t-test was used. *p < 0.05, **p < 0.01. ns, non-significance (p > 0.05).