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. 2022 Jun 15;13:874778. doi: 10.3389/fimmu.2022.874778

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Copyright © 2022 Lee, Kim and Lee

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expanded Tregs by IL-2c do not reverse established AA. (A) The experimental scheme shows that C3H/HeJ mice with AA were intradermally injected with IL-2c three times a week for 6 weeks and sacrificed on day 39. (B) Representative photos show before and after treatment. The graph shows portions of the hairless area before and after treatment (n = 6). (C) Representative images show skin Tregs of AA involved C3H/HeJ mice with or without IL-2c injections. Arrows depict Tregs. (D, E) Representative dot plots show Tregs and CD8 T cells in the skin and SDLN of AA mice with or without IL-2c injections. Results are pooled from three independent experiments. ns, non significance (p > 0.05).