Table 2.

Hepatozoon americanum copy numbers detected in cell culture aliquots removed from co-cultures of canine buffy coats with ISE6 cells.

Sample	Days in culture	Number of H. americanum copies per 5μL sample
ISE6 P/Sa 1	18	2.077 × 102
ISE6 P/S 2	18	2.442 × 102
ISE6 1	18	9.043 × 101
ISE6 2	18	1.924 × 102
ISE6 1	39	1.58
ISE6 P1b −1	8	1.32
ISE6 P/S 1 + 2c	53	7.38
ISE6 1 + 2	53	3.55

P/S, denotes addition of penicillin-streptomycin to these flasks.

^aISE6, Ixodes scapularis embryonic tick cell line (additional number after ISE6 was used to identify individual flasks, or when samples from flasks were pooled for extraction).

^bP1, denotes a passage.

^c1 + 2, denotes a pooled sample from two flasks.