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. 2022 Jun 21;13(9):2781–2797. doi: 10.7150/jca.73690

Figure 1.

Figure 1

RELA transcriptionally regulates miR-21 in PDAC cells. (A) Representative ISH images of miR-21 in the TMA containing 69 pairs of PDAC tissues and adjacent normal tissues (scale bar, 100 µm; magnification, x200). (B) Overall survival of patients with low and high miR-21 expression levels was analyzed via ISH of the TMA. (C) Kaplan-Meier curves for the overall survival probability in 178 patients with PDAC with low (n=65) and high (n=113) miR-21 expression levels. Curves were produced using a Kaplan-Meier plotter and analyzed using the log-rank test. (D) Expression levels of miR-21 in PDAC cells following transfection with si-RELA or RELA overexpression plasmid. (E) Expression level of pri-miR-21 in PDAC cells after transfection with si-RELA or RELA overexpression plasmid. (F) The expression of RELA and miR-21 were positively correlated in PDAC by TCGA database analysis. (G) DNA-binding motifs of RELA on the promoter of vacuole membrane protein 1 were predicted using the JASPAR database. (H) Chromatin immunoprecipitation-quantitative PCR assays were performed on the RELA promoter using an anti-RELA antibody in PANC-1 and MIA PaCa-2 cells. (I) Luciferase activity in PANC-1 and MIA PaCa-2 cells transfected with the pRL-TK vector and RELA overexpression vector or negative control plasmid was detected. All experiments were performed in triplicate. Data are presented as the mean ± SD. *P<0.05, **P<0.01 and ***P<0.001.