Figure 6.

ARHGAP24 suppresses cell proliferation and the cell cycle, and promotes apoptosis in pancreatic ductal adenocarcinoma. (A) EdU assays demonstrated that ARHGAP24 suppressed PANC-1 cell proliferation. (B) PI staining, followed by flow cytometric analysis, was used to analyze the cell cycle in PANC-1 and MIA PaCa-2 cells transfected with si-ARHGAP24 and the ARHGAP24 plasmid or the control. (C) Cell apoptosis was assessed using FITC-Annexin V and PI staining, followed by flow cytometric analysis, in PANC-1 cells and MIA PaCa-2 cells transfected with si-ARHGAP24 and ARHGAP24 plasmid or the control. All experiments were performed in triplicate. Data are presented as the mean ± SD. ^*P<0.05, ^**P<0.01 and ^***P<0.001.