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. 2022 Jul 1;11(1):2096362. doi: 10.1080/2162402X.2022.2096362

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — OAd-MUC16-BiTE–mediated oncolysis enhances T-cell–mediated tumor cell killing and bystander effect. (a) SKOV3-LUC/SKOV3-MUC16 or HEY-LUC/HEY-MUC16 cells were infected with OAd or OAd-MUC16-BiTE; uninfected cells were used as the control. PBMC-derived T cells (5:1) was added 24 h after infection and incubated for 24 h.(b) HEK293A cells infected with OAd or OAd-MUC16-BiTE were co-cultured with HEY-MUC16 or SKOV3-MUC16 cells and PBMC-derived T cells (5:1) for 24 h and 48 h. A co-culture without T cells served as the negative control. (a-b) Luciferase activity was measured using a microplate reader. Data represent the mean ± SD in triplicates. CON, control.