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. 2022 Jul 3;14(1):2089006. doi: 10.1080/19490976.2022.2089006

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Ethanol depletes Bt. (a) Design of Lieber-DeCarli model. (b) Serum ALT levels (Pair fed = n6; EtOH = n10). (c, d) Histological determination of hepatic steatosis with representative pictures of H&E staining (n = 5 per group). (e) Liver triglyceride content (Pair fed = n5; EtOH = n6). (f) Quantification of Bt DNA in feces measured by qPCR (n = 5 per group). (g) Quantification of Bt number (expressed in percentage) in LYBHI medium supplemented with different ethanol concentrations. Data are expressed in mean ± SEM; *p < .05, **p < .01, ***p < .001, ****p < .0001 according to two-tails student’s t-test.

Abbreviations: Bt, Bacteroides thetaiotaomicron.