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Intracellular levels of ppGpp (A) or ATP (B) and PHA content of P. oleovorans GPo1/pmPoCZ (squares), GPo500/pmPoCZ (circles), and GPo500/pBBR1MCS-2 (triangles). Cells were grown in 0.5 N E2P supplemented with 2.5 g of sodium octanoate per liter and 50 μg of kanamycin per ml. Culture samples were analyzed for ppGpp (A) or ATP (B) and PHA content at the start of PHA degradation. Each value is the result of two determinations.
