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. 2022 Mar 25;5(2):261–270. doi: 10.20517/cdr.2021.100

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© The Author(s) 2022.

© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Hsa_circ_0020714 upregulates SOX4 expression via sponging miR-30a-5p in NSCLC cells. (A) Putative miR-30a-5p binding sites to hsa_circ_0020714 and SOX4 were predicated via StarBase v3.0. (B) Hsa_circ_0020714 expression was detected in six NSCLC cell lines using qRT-PCR. (C) CircRIP analysis were carried out using an AGO2 antibody in A549 cells extract. (D) The luciferase activity of wild type (wt) or mutant (mu) pLG3-hsa_circ_0020714/SOX4 mRNA 3′-UTR in the HEK-293T cells after cotransfected with miR-30a-5p. (E) Hsa_circ_0020714 expression was modified in the NCI-H460 cells by lentivirus-mediated cDNA transfection. (F, G) SOX4 mRNA and protein expression levels in wild type (wt) or mutant (mu) hsa_circ_0020714-overexpressing NSCLC cells. ***P < 0.001, **P < 0.01, *P < 0.05. NS: Not significant.