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. 2022 Jul 5;5:666. doi: 10.1038/s42003-022-03565-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Pillar 1 SARS-CoV-2 lineages over time (B.1.1.7_n = 729, B.1.177_n = 764). The number of genomes is representative of the number of positive cases in pillar 1 at that time, which reflects the epidemic curves of the SARS-CoV-2 pandemic in the UK. b Representation of lineage composition of pillar 2 data (B.1.1.7_n = 150, B.1.177_n = 179). c sgRNA abundance in samples of lineages B.1.177 and B.1.1.7 in pillar 1 samples from the most highly expressed ORFs. d sgRNA abundance in samples of lineages B.1.177 and B.1.1.7 in pillar 2 samples from the most highly expressed ORFs. e E gene cycle threshold (ECT) for B.1.177 (n = 257) and B.1.1.7 (n = 185) lineages. f Relative light units (RLU) for B.1.177 (n = 626) and B.1.1.7 (n = 626) lineages. g E gene cycle threshold compared to total raw sgRNA counts (high and low quality) for B.1.177 and B.1.1.7. Correlation coefficient and p value using Pearson. h E gene cycle threshold compared to total raw genomic RNA counts for B.1.177 and B.1.1.7. Correlation coefficient and p value using Pearson. i Raw sgRNA counts normalised to E gene cycle threshold (sgRNA*ECT). j Protein abundance for S M and N proteins measured by LC-MS/MS and normalised to ORF1a in B and B.1.1.7 infected TMPRSS2 & ACE2 expressing A549 cells at 12 and 24 h post infection (n = 3 independent experiments). All p values (except g, h, j) calculated using an unpaired Wilcoxon signed-rank test and adjusted for multiple testing with the Holm method (**** <0.0001, *** <0.001, ** <0.01, * <0.05). All boxplots depict the 25th, 50th (median), and 75th percentiles, and whiskers represent the most extreme datapoint which is no more than 1.5x the interquartile range. B.1.1.7 is represented in red, B.1.177 in teal, B in yellow, B.1 in green, B.1.1 in navy blue, B.1.1.1 in purple and other lineages in grey.