FIG. 1.
Composition of pTGFP2. A928-bp EcoRI-SphI fragment encompassing tetR, Ptet, and the E. coli atpE translation initiation region (in black), all obtained by PCR, was inserted into pJBA27. The bla gene encodes β-lactamase conferring resistance to ampicillin. ori colE1, origin of replication originating from pUC18-NotI; lacZ′, partially deleted lacZ gene; gfpmut3, FACS-optimized gfp gene encoding GFP. The two T's are transcriptional terminator sequences from pJBA27. Ptet is the tet promoter and tetR encodes the tet repressor protein, both originating from Tn10.