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DNA damage in PBMC isolated by Ficoll-Paque and in the respective cell subpopulations: cHPSC CD34+/CD3-/CD14-, T lymphocytes CD3+, monocytes CD14+. Representative FACS analysis of γ-H2AX dot plots of the different studied groups (40 frail, 13 pre-frail, 32 non-frail, 46 young samples). Histograms represent the percentage of cells positive for DNA damage (γ-H2AX) in the different groups. SSC: side scatter. Data are expressed as means ± SEM. *p < .05.
