Table 2.
Ficoll-Paque Isolated PBMC and the Respective Mononucleated Cell Subpopulations Analyzed by FACS With the Gating Strategy Shown in Supplementary Figure 1
| Frail | Pre-Frail | Non-Frail | Young | |
|---|---|---|---|---|
| N = 40 | N = 13 | n=32 | n=46 | |
| PBMC | ||||
| n* | 800 503 ± 784 393 | 942 967 ± 763 245 | 846 580 ± 696 317 | 1 068 624 ± 766 984 |
| 474 416 | 812 920 | 595 650 | 807 084 | |
| (205 072–1 168 448) | (343 009–1 315 235) | (286 251–1 321 378) | (400 997–1 856 148) | |
| T cells CD3+ | ||||
| % | 57.4 ± 14.1 | 59.2 ± 17.7 | 59.1 ± 9.5 | 62.2 ± 12.0 |
| 58.6 | 66.2 | 56.6 | 62.6 | |
| (50–69) | (49–72) | (52–67) | (56–70) | |
| Cells CD3−/CD14− | ||||
| % | 33.8 ± 12.0 | 32.2 ± 17.8 | 33.2 ± 9.7 | 31.1 ± 12.0 |
| 33.5 | 24.0 | 34.7 | 29.4 | |
| (24–40) | (17–40) | (25–39) | (23–35) | |
| Monocytes CD14+ | ||||
| % | 7.0 ± 4.6 | 5.9 ± 3.5 | 6.3 ± 4.3 | 5.5 ± 3.5 |
| 6.6 | 5.5 | 5.0 | 4.5 | |
| (4–8) | (7–3) | (3–7) | (3–7) | |
| cHPSC CD3−/CD14−/CD34+ | ||||
| % | 0.076 ± 0.070 | 0.099 ± 0.068 | 0.068 ± 0.040 | 0.081 ± 0.072 |
| 0.062 | 0.092 | 0.05 | 0.07 | |
| (0.06–0.09) | (0.03–0.14) | (0.04–0.09) | (0.03–0.10) |
Notes: PBMC = peripheral blood mononuclear cell. Samples obtained from 85 older adults divided according to the frail, pre-frail, non-frail status and from a group of 46 young subjects. Values are expressed as mean ± SD and median (first–third quartile). Statistical analysis was not significant among all groups.
*PBMC were analyzed with the non-parametric Wilcoxon–Mann–Whitney test.