Figure 3.

A: concentration-dependent increase in [Ca²⁺]_i in mVSMCs in response to extracellular zinc (n = 5 for each concentration). B: dose-dependent increase in mVSMC [Ca²⁺]_i in response to 15-HETE with and without zinc (n = 5–7 for each concentration). C: summary of [Ca²⁺]_i response to 1 μM 15-HETE in mVSMCs treated with a lentivirus containing either a scrambled or GPR39-targeting shRNA for 72 h (n = 5, *P = 0.0122). D: summary of changes in mVSMCs [Ca²⁺]_i in response to 50 nM of 14,15-EET and 15-HETE, separately and in combination, and with and without zinc (n = 4 for zinc alone, and n = 6). When applied alone, neither 4 μM zinc nor 50 nM 15-HETE had an effect on [Ca²⁺]_i. Zinc (4 μM) potentiates the effect of 15-HETE (50 nM) on [Ca²⁺]_i in mVSMCs, and the increase in mVSMCs [Ca²⁺]_i by 15-HETE is abolished by pretreatment with 14,15-EET (*P < 0.00011). EET, epoxyeicosatrienoate; GPR39, G protein-coupled receptor 39; HETE, hydroxyeicosatetraenoate; mVSMCs, microvascular smooth muscle cells.