Figure 1.

CMV-cre/Ptpra^f/f (Ptpra^−/−) mice do not express protein tyrosine phosphatase-α (PTPα). A: tail clips from CMV-cre/Ptpra^f/f (Ptpra^−/−) mice were genotyped by endpoint PCR for the Ptpra allele. Genotyping results for two representative mice are shown on the left (1 and 2), with control alleles for the recombinant (RC), wild type (WT) and floxed alleles shown on the right. B: isolated lung fibroblasts from Ptpra^−/− mice were lysed for Western blot analysis of expression of PTPα protein. WT and Ptpra^−/− murine embryonic fibroblasts (MEFs) are shown as positive and negative controls.