Figure 4.
Profibrotic gene expression is reduced in the absence of Ptpra. Ptprawt/wt and Ptpra−/− mice were treated with 0.19 N HCl or saline and harvested at 48 h. A: mRNA expression of fibrotic markers Eda-fibronectin, Ctgf, and Acta2 was assessed in homogenized lung tissue using qPCR. Right-skewed variables were natural-log transformed prior to analysis for significance. Each symbol represents an individual mouse and horizontal lines represent geometric mean and 95% confidence interval (CI). Values in each group represent results from pooled independent experiments with a total of 4–7 mice/group. *P < 0.05; t test between specific treatment combinations. B: a fibrosis-focused qPCR array was performed on homogenized lung tissue. Data were normalized to the housekeeping gene GusB. qPCR array plates were performed in triplicate and data are represented as means ± SE. *P < 0.05.